Yuav Ua Li Cas Extract Luteolin?

Luteolin (3,4,5,7-tetrahydroxyflavonoid) yog lub ntuj flavonoid, muaj npe tom qab nws tau pib cais tawm ntawm luteolin Reseda odorata Linn. hauv tsev neeg Oleaceae. Nws tuaj yeem pom los ntawm cov qauv uas luteolin yog polyphenolic hydroxyl compound nrog lipophilicity tsis zoo. Vim yog lub zog sib xyaw ua ke ntawm cov pab pawg hydroxyl, lub lattice zog yog siab, thiab nws cov hydrophilicity kuj tsis zoo. Vim nws cov qauv tshwj xeeb, Cov solubility tsawg hauv ntau cov kuab tshuaj ua rau muaj kev nyuaj rau nws cov kev npaj thiab kev siv.

Yuav ua li cas extract luteolin?

Cov txheej txheem rho tawm

Kev siv tshuab thev naus laus zis ntawm luteolin tuaj yeem muab faib ua ob pawg, ib txwm siv thev naus laus zis thev naus laus zis, thiab thev naus laus zis tshiab. Cov thev naus laus zis tshiab tuaj yeem muab faib rau hauv kev rho tawm ncaj qha thiab rho tawm tom qab raw khoom ua ntej. Cov qub muaj xws li txoj kev ultrasonic-pab, microwave-pab txoj kev, thiab supercritical kua txoj kev. Txoj kev rho tawm thiab ionic kua synergistic extraction method; tom kawg muaj ib txoj kev enzyme-pab thiab chav tawg pretreatment txoj kev, thiaj li yuav tau luteolin nyob rau hauv siab yield thiab ruaj khov zoo.

1 Txheej txheem extraction

Cov txheej txheem tshem tawm cov kuab tshuaj yog ib txoj kev rho tawm ntau dua, uas tau muab faib rau hauv txoj kev immersion, percolation method, water decoction method, reflux extraction method, thiab nruam extraction method. Tam sim no, txoj kev rho tawm feem ntau siv rau luteolin yog reflux extraction, thiab cov kuab tshuaj feem ntau yog 60 feem pua ​​​​rau 80 feem pua ​​​​methanol lossis ethanol.

Hauv cov ntawv tshaj tawm txog kev tshem tawm cov kuab tshuaj, qee cov kws tshawb fawb tau rho tawm luteolin los ntawm cov nroj tsuag tshuaj xws li Vitex Chinensis, blackberry, Du Honghua, Prunella vulgaris, thiab Duyiwei, thiab cov extraction tus nqi yog 1 feem pua ​​​​rau 3 feem pua.

2 Ultrasound-pab txoj kev

Ultrasonic-pab txoj kev siv lub high-speed, muaj zog cavitation nyhuv thiab stirring nyhuv generated los ntawm lub suab yoj, yog li rhuav tshem cov cellular qauv ntawm cov ntaub ntawv raw, accelerating tus nkag mus rau cov hnyav, shortening lub sij hawm extraction, thiab txhim kho tus nqi extraction. . Qhov zoo ntawm txoj kev no yog: (1) siab extraction efficiency; (2) Lub sijhawm luv luv; (3) qhov kub thiab txias; (4) dav siv; (5) tsawg impurities nyob rau hauv cov extract, uas yog conducive rau kev sib cais thiab purification; (6) rho tawm Cov txheej txheem ua haujlwm raug nqi tsawg; (7) kev ua haujlwm yog yooj yim, thiab cov cuab yeej tu thiab tu yog yooj yim

3 Microwave-pab txoj kev

Microwave-pab extraction yog cov cuab yeej tshiab rau kev rho tawm cov khoom siv hluav taws xob microwave. Kev tiv thaiv ib puag ncig, kev siv dav dav, thiab lwm yam ntxwv. Lub ntsiab tseem ceeb ntawm cov txheej txheem yog kev xaiv cov kuab tshuaj, kua-cov khoom sib piv, lub sijhawm hluav taws xob microwave, lub zog hluav taws xob microwave, qib ntawm fragmentation, kev cuam tshuam ntawm cov dej los yog cov av noo hauv cov khoom, thiab lwm yam uas cuam tshuam rau microwave extraction (strengthening) .

4 Supercritical kua extraction

Supercritical kua extraction yog hais txog kev sib cais ntawm kev rho tawm cov khoom siv los ntawm cov khoom lossis cov kua siv cov kua supercritical ua kuab tshuaj. Supercritical fluid muaj cov pa roj zoo li diffusion coefficient, kua dissolving zog, thiab qhov nro ntawm 0. Nws tuaj yeem nkag mus rau hauv cov khoom siv sai thiab tshem tawm cov ntsiab lus ntawm nws cov khoom. Nws muaj cov yam ntxwv ntawm kev ua haujlwm siab, tsis muaj oxidation, ntuj ntshiab, thiab tsis muaj tshuaj lom neeg.

5 Synergistic extraction ntawm ionic kua

Cov kua dej ionic xa mus rau cov ntsev uas yog kua ntawm los yog ze ntawm chav tsev kub thiab muaj tag nrho cov anions thiab cations, tseem hu ua low-temperature molten salts. Hauv kev tshawb fawb tam sim no, cov cations feem ntau yog imidazolium cations, thiab cov anions feem ntau yog halogen ions thiab lwm yam inorganic acid ions (xws li tetrafluoroborate, thiab lwm yam). Nws muaj cov yam ntxwv zoo xws li tsis tshua muaj siab vapor siab, txoj kev ua kua dav, muaj zog solubility, thiab tuaj yeem tsim thiab hloov kho, thiab nws cov khoom siv rov ua dua tshiab yog tus yuam sij los ua cov kuab tshuaj ntsuab, thiab nws muaj cov ntawv thov dav dav.

6 Txoj Kev Pabcuam Enzyme

Lub hauv paus ntsiab lus ntawm cov enzyme-pab extraction txoj kev yog siv lub siab tshwj xeeb ntawm cov tshuaj tiv thaiv enzyme, xaiv cov enzyme sib xws, hydrolyze los yog degrade lub Cheebtsam ntawm lub cell phab ntsa, rhuav tshem nws cov qauv, thiab ua rau cov encapsulated active cov khoom xyaw ncua los yog yaj nyob rau hauv lub kuab tshuaj. , cov enzyme yog yooj yim inactivated, thiab cov tshuaj tiv thaiv tej yam kev mob yuav tsum yog me me. Txoj kev no tuaj yeem txhim kho tus nqi rho tawm, thiab cov txiaj ntsig cuam tshuam feem ntau yog hom enzyme, enzymatic hydrolysis lub sijhawm, kub, pH tus nqi, thiab lwm yam.

7 Steam explosion pretreatment method

Chav tawg pretreatment yog ib qho kev tawm dag zog lub cev pretreatment technology, uas tuaj yeem txo qhov degree ntawm cellulose polymerization, ib nrab degrade hemicellulose, soften lignin, thiab txo cov transverse bonding zog. Cov nyhuv kab rov tav ntawm ib txhij nce nws qhov chaw saum npoo thiab qhov porosity ua rau muaj peev xwm txhim kho cov enzymes zom cov cellulose. Cov kev tshawb fawb tau pom tias cov cuab yeej no tuaj yeem txhim kho qhov txiaj ntsig ntawm cov khoom siv raw, thiab cov cuab yeej no tau siv dav hauv cov roj, nplej, thiab suav tshuaj ntsuab kev lag luam.

8 Txoj kev sib koom tes rho tawm

Txoj kev extraction synergistic yog hais txog kev siv ob lossis ntau txoj hauv kev los sib koom ua ke cov ntaub ntawv raw kom ua tiav cov txiaj ntsig zoo ntawm cov txheej txheem txhawm rau kom tau txais cov txiaj ntsig zoo tshaj plaws extraction.

Muaj ntau yam kev sib txuas ntawm txoj hauv kev sib koom ua ke. Nyob rau hauv cov ntaub ntawv ntawm kev rho tawm ntawm luteolin, qee cov kws tshawb fawb siv ultrasonic synergistic microwave extraction, ultrasonic synergistic enzymatic extraction, ionic kua synergistic microwave-pab extraction, thiab ionic kua synergistic ultrasonic extraction. Cov khoom siv raw siv muaj brocade lanterns, vines, txiv laum huab xeeb plhaub, pob kws txhob lo lo ntxhuav, thiab lwm yam. Vim muaj cov ntaub ntawv sib txawv, tus nqi rho tawm ntawm luteolin yog 0.1 feem pua ​​rau 0.3 feem pua. Los ntawm kev sim cov txiaj ntsig, txoj kev sib koom ua ke muaj kev txhim kho ntau dua hauv kev rho tawm tus nqi ntau dua li cov txheej txheem ib txwm muaj. Piv nrog rau ib txoj kev tshiab, cov nyhuv txawv, thiab feem ntau yog cov txiaj ntsig zoo. Txawm hais tias kev ua haujlwm ntawm txoj kev sib koom ua ke extraction yog qhov nyuaj, qhov kev ua kom zoo ntawm cov extraction yog siab thiab tuaj yeem ua tiav ib leeg qhov zoo. , nrog kev txhim kho thiab kev tshawb fawb zeem muag.

Yog tias koj xav paub ntau ntxiv txog luteolin, thov hu rau peb ncaj qha ntawmmedical@ysgcn.com